Preparation of adeno-associated virus serotype 8 using baculovirus system / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 134-138, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-840095
ABSTRACT
Objective:
To set up a baculovirus system for preparing recombinant adeno-associated virus serotype 8 (rAAV8) and to preliminarily examine the infection viability of the prepared virus in vitro.Methods:
The rAAV8-EGFP was prepared using baculovirus system and was subsequently extracted and purified by high performance liquid chromatography. The viral titer was determined by real-time quantitative PCR and its infection viability for HEK-293 cells was examined by observing the EGFP reporter.Results:
Real-time quantitative PCR showed that high titer of rAAV8-EGFP was prepared (1.5×10 13vg/bottle[100 ml medium]) and strong expression of EGFP was observed in HEK-293 cells infected with rAAV8-EGFP.Conclusion:
The baculovirus system is capable of producing rAAV8 with high titer and infection viability, paving a way for future research.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2011
Tipo de documento:
Artigo
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