Optimization of extraction process for phenolic glycosides from Rhizoma Curculigins by orthogonal test / 第二军医大学学报

ABSTRACT

Objective To optimize the extraction process for phenolic glycosides from Rhizoma Curculigins. Methods The contents of orcinol glucoside,curculigoside and curculigine were used as parameters, and they were determined by HPLC. The extraction conditions of phenolic glycosides from Rhizoma Curculigins were optimized using orthogonal design. The examined factors included concentration of ethanol(50% , 70% , and 95%) , extraction time (1, 1.5, and 2 h) , times of extraction (1,2, and 3) , and solvent volume (6, 8, and 10 times). Results The concentration of ethanol, extraction time, times of extraction and solvent volume significantly affected the extraction efficacy of phenolic glycosides in Rhizoma Curculigins, with the most prominent effect exerted by the ethanol concentration. The optimal extraction process was refluxing three times with 70% ethanol (10 times of solvent volume), for 2 h each time. Conclusion The present method can effectively extract phenolic glycosides from Rhizoma Curculigin, which paves a way for further research on phenolic glycosides in Rhizoma Curculigins.