Physicochemical properties and biological activity of a recombinant inducible co-stimulator-Ig fusion protein / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 128-131, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-840367
ABSTRACT
Objective:
To investigate the physicochemical properties and biological activity of self-prepared fusion protein inducible co-stimulator-Ig.Methods:
Acid hydrolysis, edman degradation and peptide mass finger printing were used to determine the amino acid composition, N-terminal 15 amino acid sequences, and peptide mapping. In vivo mixed lymphocyte reaction assay was used for identification of its biological activity.Results:
The result of amino acids composition analysis was consistent with the theoretical value of ICOS-Ig. N-terminal 15 amino acid sequences of the product were EINGSANYEMFIFHN, consistent with the theoretical value of ICOS-Ig. Peptide match assay identified six peptides of the product which could match the theoretic maps of ICOS-Ig. ICOS-Ig and CsA noticeably inhibited the proliferation of allo-reactive T cells in vivo.Conclusion:
The prepared ICOS-Ig fusion protein has a correct structure and can inhibit the proliferation of allogeneic T cells in vivo, which lays a foundation for quality control of ICOS-Ig fusion protein.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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