Reversed-phase ion-pair chromatography in determination of sinomenine contents in Sinomenium acutum / 第二军医大学学报

ABSTRACT

Objective: To establish a reversed-phase ion-pair chromatography (RP-IPC) method for determination of sinomenine content in Sinomenium acutum. Methods: The IPC method was established using a column of Eclipse Plus C18(4. 6 mmX250 mm, 5 μm) with a mobile phase of acetonitrile and buffer phosphate (0. 03 mol/L potassium dihydrogen phosphate and 0. 03 mol/L sodium heptane sulfonic acid, adjusted to pH 4. 0 by phosphoric acid) 18 82. The flow rate was 1. 0 ml/min; the detection wavelength was set at 262 nm, and the temperature was at 40°C. Results: The linearity was obtained over a concentration range of 0. 025-0. 5 mg/ml(r=1. 000). The low limit of detection was 2. 5 μg/ml. The intra-day precisions of low, medium, and high concentrations of sinomenine were 1.54%, 0. 70%, and 0. 45%, and the inter-day precisions were 1. 54%, 0. 70%, and 0. 45%, respectively. The average recovery was 99. 9%(RSD=2. 9%, n=6), which met the requirement for analysis. Conclusion: The present method is simple, fast, and accurate; it is suitable for determination of sinomenine content in Sinomenium acutum.