Effects of α-lipoic acid on cerebral IRE1 expression induced by sleep deprivation in rats / 第二军医大学学报

ABSTRACT

Objective: To observe the influences of sleep deprivation and antioxidant agent α-lipoic acid on the expression of IRE1 in the cortex and hippocampus in rats. Methods: Rats were randomly divided into control groups and sleep deprivation group. The control groups included blank control group (n=10) and environmental control group (n=10). Sleep deprivation group included 120 rats, and rats were further divided into α-lipoic acid group (25 mg.kg-1.d-1) and sleep deprivation model group. The two were further divided into 6 groups according to the periods of sleep deprivation and sleep restoration (SD 1 d , SD 3 d, SD 5 d, SD 7 d, SD 7 d/RS 6 h, SD 7 d/RS 12 h[n=10]). The expression of IRE1 mRNA and protein was examined by RT-PCR and immunohistochemistry, respectively. Results: RT-PCR results showed that the expression of IRE1 mRNA in the cortex and hippocampus were significantly higher in sleep deprivation group than in the blank and environmental control groups (P<0.05); there was no significant difference between the latter 2 groups. α-lipoic acid decreased the expression of IRE1. Expression of IRE1 between α-lipoic acid groups and sleep deprivation model groups was significantly different on SD 5 d, SD 7 d, SD 7 d/RS 6 h and SD 7 d/RS 12 h(P<0.05). The imrnunohistochemistry results of IRE1 expression were consistant with RT-PCR results. Conclusion: α-lipoic acid can reduce the cerebral IRE1 expression in sleep deprivation rats, which provides a basis for antioxidant treatment of sleep deprivation.