Screening and identification of HLA-A0201 restricted CTL epitope from hepatitis B virus X protein in vitro / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 1169-1174, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-841001
ABSTRACT
Objective:
To identify the HLA-A0201 restricted CTL epitopes derived from hepatitis B virus X protein predicted by computer program and general principles in vitro.Methods:
HBx gene sequences of Hepatitis B virus genotypes B/C and serotypes adw/adr, with the highest frequencies in Chinese, were computed and analyzed by screening service offered by Internet combined with peptide supermotif, extended motif and quantitative motif prediction. Four most ideal nine-peptides (HBx1, HBx2, HB3x3, and HBx4) were selected as candidate peptides. Using flow cytometry, the fluorescence index of both control and experimental groups were detected and the 4 nine-peptides were evaluated with T2 binding assay and DC50 assay.Results:
The nine-peptides VLCLRPVGA (HBx1), CLFKDWEEL (HBx2), VLHKRTLGL (HBx3) and HLSLRGLPV (HBx4) were selected as candidate targets. Among the 4 candidate peptides, HBx2 showed higher HLA-A0201 affinity and HBx2, HBx4 showed better stability.Conclusion:
Our study indicates that CLFKDWEEL might be a potential HLA-A0201 restricted CTL epitope from hepatitis B virus X protein; further study is needed for verification of its immunity in vivo.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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