Effect of low intensity pulsed ultrasound on osteogenic potential of rabbit bone marrow stromal cells in vitro / 第二军医大学学报

ABSTRACT

Objective: To explore the effect of low intensity pulsed ultrasound (LIPUS) on the osteogenesis of rabbit bone marrow stromal cells (BMSCs) in vitro. Methods: Rabbit bone marrow was drawn out and cultured with conditioned culture medium to harvest BMSCs; the third generation BMSCs were randomly assigned to the experimental group and the control group. The experimental group was exposed to LIPUS 20 min/day for 1, 2 or 3 weeks, and the control group received no treatment. The cell proliferation was observed under inverted microscope. Von Kossa staining and alkaline phosphatase (ALP) activity test were employed to assess the osteogenesis of the cells. Results: BMSCs became larger after being stimulated with LIPUS, with increased nuclei and decreased nuclear to cytoplasm ratio; BMSCs untreated with LIPUS was flat and had lower refractive index. ALP activities of LIPUS-treated (1, 2, and 3 weeks) BMSCs increased significantly compared with that of the control group(P<0.05); the pixel value of calcification nodule in experimental group(after 3 weeks) was also higher than that of the control group(P<0.05). Conclusion: LIPUS can accelerate the osteogenesis of rabbit BMSCs in conditioned culture medium.