Recombinant adeno-associated virus mediated RNA interference of angiogenin inhibits growth of human lung squamous cancer cells / 第二军医大学学报

ABSTRACT

Objective: To inhibit the expression of angiogenin (ANG) by recombinant adeno-associated virus (AAV) mediated RNA interference (RNAi) and to observe its influence on the growth of human lung squamous cancer cells line, SK-MES-1. Methods: A recombinant adeno-associated virus vector was constructed and used to deliver small hairpin RNA (shRNA) targeting ANG into SK-MES-1 cells; SK-MES-1 cells and SK-MES-1 cells transfected with AAV-Null were taken as control. The inhibitory effect of adeno-associated virus mediated RNAi on ANG expression and subsequent influence on the growth, tumorigenesis, and microvessel density of SK-MES-1 tumors were also evaluated. Results: In vitro experiment showed that AAV-shANG was successfully constructed. Western blotting revealed that AAV-shANG effectively infected SK-MES-1 cells and the expression of ANG was obviously decreased, 72 h after transduction. In vivo study showed that, compared with the other 2 groups, the suppression of ANG in AAV-shANG group significantly inhibited the growth of SK-MES-1 cells and decreased the MVP in nude mice (P<0. 05). Conclusion: The constructed AAV-shANG can effectively inhibit ANG protein expression in SK-MES-1 cells, which paves a way for the therapy of human lung squamou cancer.