Expression of complement regulatory protein C1-INH and CD46 in nasal mucosa of allergic rhinitis rats / 第二军医大学学报

ABSTRACT

Objective: To determine the expression of complement regulatory proteins (CRPs) C1-INH, MCP (CD46) in the nasal mucosa of allergic rhinitis rats and to study the role of CRPs in the pathogenesis of allergic rhinitis. Methods: Nine healthy SD rats were sensitized and intranasally challenged with ovalbumin and Al(OH)3 (as supplement) to establish allergic rhinitis models and another 9 SD rats treated with saline were taken as control. The nasal mucosa in respiratory area of both groups was obtained. Then Western blotting was performed to investigate the expression levels of C1-INH and CD46. Results: Western blotting showed that both C1-INH and CD46 had been detected in rat nasal mucosa. Expression level of CD46 in the nasal mucosa of allergic rhinitis was significantly higher than that in control rats(P<0.01); whereas the level of C1-INH was lower than that in control rats(P<0.05). Conclusion: It is suggested that both C1-INH and CD46 maybe involved in the pathogenesis of rat allergic rhinitis. The regulatory role of CRPs may be related to the classical pathway of complement activation.