Myeloma protein-pusled dendritic cells induced anti-myeloma cells activity / 第二军医大学学报

ABSTRACT

Objective: To investigate the anti-myeloma cells specific immune activity induced by myeloma protein-pusled dendritic cells(DCs). Methods: DCs were obtained from peripheral blood(PB) of multiple myeloma(MM) patients after co-culture with GM-CSF, IL-4 and TNF-α. The phenotype of DCs was examined by flow cytometry. Host lymphocytes were stimulated with DCs pulsed with myeloma protein acquired from serum of MM patients on DEAE-celluose; lymphocytes treated with unpulsed DC served as control. [3H]-thymidine incorporation and 4 h 51Cr-release assay were used to detect the proliferation and cytotoxicity against different targets(auto myeloma cells, U266 cells) by T lymphocytes. Results: In vitro proliferation and cytotoxicity of T lymphocytes activated by myeloma protein pulsed-DCs were greater than those of T lymphocytes in the control group(P<0.01). T lymphocytes in neither of the 2 groups had notable cytotoxicity against U266 cells. Conclusion: DCs pulsed by myeloma protein can effectively induce specific cytotoxicity of T lymphocytes against myeloma cell.