Inhibition of xathine oxidase by renierol extracted from South China Sea sponge / 第二军医大学学报

ABSTRACT

Objective: To study the inhibition of xanthine oxidase by renierol extracted from South China Sea sponge and study the influence of renierol on hyperuricemia in mice. Methods: After extracted from South China Sea sponge, renierol (20, 40, 60 μg/ml) was added to a system containing xanthine oxidase (0. 1 μ/ml) and xanthine (50 μmol/ml); allopurinol (1 μg/ml) was also addded to the system as positive control. The 5 min-fomation of superoxide anions was used to determine the activity of xanthine oxidase (Nitro Blue Btetrazolium reduction). Renierol (20, 40, 60 μg/ml) was added to 25°C pre-heated pyrogallol autoxidation to observe its eliminating effect on free radicals through determining the absorbance rate at 420 nm wavelength. Potassium oxonate, a uricase inhibitor, was used to induce hyperuricemia in mice and the mice were then treated with oral renierol (10, 20, 30 mg · kg-1) or allopurinol (2 mg/kg) as positive control. The decrease of serum uric acid induced by renierol was determined by automatic biochemical analyzer. Results: Renierol was demonstrated to be a competitive inhibitor of xanthine oxidase in vitro , with its IC50 value being 1.36 μg · ml-1. It also decreased uric acid in vivo. Conclusion: Renierol can decrease serum uric acid through inhibiting the xanthine oxidase.