Inhibitory effect and apoptosis induction of MST1R inhibitor BMS-777607 on proliferation of breast cancer MCF-7 cells / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the effects of macrophage stimulating 1 receptor (MST1R ) inhibitor BMS-777607 on the proliferation and apoptosis of the breast cancer MCF-7 cells, and to elucidate the mechanisms. Methods: The breast cancer MCF-7 cells treated with different concentrations of BMS777607 were divided into control group (0/xmol • L BMS-777607 group) and 0. 5, 1.0» 2.0, 5. 0, 10.0. 15. 0. and 20.0/xmol • L BMS-777607 groups. MTT method was used to detect the proliferation rates of the MCF-7 cells in various groups, and clone formation assay was used to detect the survival rates of the MCF-7 cells in various groups; EDU imaging and EDU flow cytometry methods were used to detect the proliferation rates of the MCF-7 cells in various groups, and Hoechst33342 staining was used to detect the apoptotic morphology of the MCF-7 cells in various groups; flow cytometry was used to detect the apoptotic rates of the MCF-7 cells in various groups∗ and Western blotting method was used to detect the expression levels of ERK, p-ERK, Akt, p-Akt, PARP, Cleaved PARP, Bax, Caspase-3, Cleaved Caspase-3, Caspase-9 and Cleaved Caspase-9 proteins in the MCF-7 cells in various groups. Results: The MTT results showed that compared with control group, the proliferation rates of the MCF-7 cells in 5 and 10/imol • L BMS-777607 groups were increased (P<0. 05 or P<0.01). The clone formation assay results showed that compared with control group, the survival rates of the MCF-7 cells in 5 and 20/imol • L IiMS-777607 groups were increased (P<0. 05 or Pcontrol group, the proliferation rates of the MCF-7 cells in 10 and 20/zmol • L BMS-777607 groups were increased ( P<∗0. 05 or P< 0.01). The Hoechst33342 fluorescence staining results showed that the MCF-7 cells in control group showed the light staining, a small number of MCF-7 cells in 10/imol • L BMS-777607 group showed the bright nuclei, and most of the MCF-7 cells in 20/imol • L BMS-777607 group showed the bright nuclei. The flow cytometry results showed that compared with control group, the apoptotic rates of the MCF-7 cells in 10 and 20