Inhibitory effect of mitochondrial dynamics changes on growth of transplanted melanoma in APP/PS1 mice / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the inhibitory effect of mitochondrial dynamics changes on the tumor growth of transplanted melanoma in the APP/PS1 the mice and its mechanism. Methods: The male C57BL/6J (C57) and APP/PS1 mice were divided into C57 transplantation tumor group (n = 7) and APP/PS1 transplantation tumor group (n = 7). The tumor appearance time was observed and the tumor volume of the mice in two groups were calculated and the growth curves of the mice in two groups were drawn. The morphology of tumor tissue of the mice in two groups was observed under light microscope, the expression levels of mitofusion 2 (Mfn2), dynamin related protein (Drpl), fission 1 (Fis1), ubiquitin and polyubiquitin, LC3- II, PTEN induced putative kinase 1 (PINK1) and Parkin proteins in tumor tissue of the mice in two groups were detected by Western blotting method. Results: The tumor tissue under the skin of the mice in C57 transplantation tumor group and APP/PS1 transplantation tumor group was found with the melanoma granules in the tumor cells. Compared with C57 transplantation tumor group, the tumor appearance time of the mice in APP/PS1 transplantation tumor group was late, and the tumor volume was decreased (P < 0. 0 5); the expression level of Mfn2 protein in tumor tissue of the mice was decreased (P < 0. 0 5); the expression levels of Drpl and Fisl proteins were increased (P < 0. 0 5), and the expression levels of ubiquitin and polyubiqitin, LC3- II, PINK1 and Parkin proteins in tumor tissue of the mice in APP/PS1 transplantation tumor group were all increased (P < 0. 0 5). Conclusion: The growth of tumor in the APP/PS1 mice with transplanted melanoma is slow, and its mechanism may be related to PINK1/Parkin pathway involved in the mitochondrial autophagy.