Effect of herba arleniisiae eapillaris extracts on pten protein expression in kidney tissue of diabetic rats and its protective effect on kidney / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the effect of herba artemisiae eapillaris extracts (HACE) on the expression of phosphatase and tensin homologue deleted chromatosome 10 (PTEN) in the kidney tissue of the diabetic rats, and to clarify the pharmacological mechanism of protective effect of HACE on the kidney of the diabetic rats. Methods: Thirty Wistar rats were randomly divided into control group (/i=6). model group ( n= 12) and HACK group (n=12). The rats in HACK group were intragastrically administrated with HACK (5 g • kg !) one time every day. and the rats in control group and model group were intragastrically administrated with the same volume of normal saline. The diabetic rat models were duplicated by streptozotocin (STZ). After 4 months of administration, the urinary albumin excretion rates and urinary total protein excretion rates of the rats in various groups were detected. The pathomorphology of the kindey tissue of the rats in various groups were observed by HE staining and the distribution of renal extracellular matrix (ECM) was detected by PAS and Mas son staining. The expressions of PTEN protein in kidney tissue of the rats in various groups were detected by immunohistostaining and the expression levels of PTEN protein in kidney tissue of the rats in various groups were detected by Western blotting method. Results: Compared with model group, the glomerular mesangial aggregation, positive staining of PAS as well as Masson aggregation and basement membrane thickening of the rats in HACE group were significantly reduced, and the 24 h urinary albumin excretion rate was significantly decreased ( P0. 05). The immunohistochemistry staining results showed that the expression level of PTEN protein in kidney tissue of the rats in control group was higher, the expression level of PTEN protein in the kidney tissue of the rats in model group was decreased, and the expression level of PTEN protein in kidney tissue of the rats in HACE group was increased to normal. The Western blotting results showed that the expression level of PTEN protein in the renal cortex tissue tissue of the rats in model group was significantly decreased compared with control group C P<0. 05) ; compared with model group, the expression level of PTEN protein in renal cortex tissue of the rats in HACE group was significantly increased C P<-0. 05). Conclusion: HACE can increase the expression of PTEN protein in kidney tissue of the rats, which may be one of the mechanisms of its protective effect on the kidney in the diabetic rats.