Effects of microspherule protein 1 on invasion and migration of gastric cancer cells and their mechanisms / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the effects of overexpression of microspherule protein 1 (MCRS1) in the gastric cancer cells on the invasion and migration of gastric cancer cells, and to elucidate their possible mechanisms. Methods: The gastric cancer BGC-823 cells, SGC-7901 cells and the normal gastric mucosal epithelial GES-1 cells were cultivated. Western blotting method was used to detect the expressions of MCRS1 in three kinds of cells. The result showed that MCRS1 protein had the lowest expression in the BGC-823 cells, so the gastric cancer BGC-823 cells were selected for next experiments. The recombinant plasmid of MCRS1 was constructed, and the gastric cancer BGC-823 cells in the logarithmic growth phase were selected. Blank group, empty vector transfection group and MCRS1 transfection group were established, and the plasmid was transfected into the BGC-823 cells using Lipo3000. The expression levels of epithelial cadherin (E-cadherin) protein, neuronal cadherin (N-cadherin) protein and Snail protein were detected by Western blotting method. The cell scratch assay and Transwell assay were used to detect the migration and invasion of gastric cancer BGC-823 cells. Results: Compared with the normal gastric epithelial GES-1 cells, the expression level of MCRS1 protein in the gastric cancer BGC-823 cells was decreased (P < 0 . 01), but the expression level of MCRS1 protein in the SGC-7901 cells was increased (P < 0 . 01). The PCR identification and sequencing analysis showed that the MCRS1 recombinant plasmid was successfully constructed. Compared with blank group and empty vector transfection group, the expression levels of MCRS1 protein and E-cadherin protein in MCRS1 transfection group were increased (PN-cadherin and Snail proteins were decreased (P < 0 . 01), the cell migration rate was significantly reduced (P < 0 . 01), and the number of invasion cells was significantly decreased (P < 0 . 01).

Conclusion:

Overexpression of MCRS1 can inhibit the migration and invasion of gastric cancer BGC-823 cells, which may be related to the increased expression of E-cadherin protein and the decreased expressions of N-cadherin and Snail proteins.