Effects of Jiawei Danshen Yin on cardiac function and serum inflammatory factors IL-6 and TNF-α in rats with chronic heart failure / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the effects of Jiawei Danshen Yin on the cardiac function and serum inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor-a (TNF-α) in the rats with chronic heart failure, and to elucidate the mechanism of Jiawei Danshen Yin in the treatment of chronic heart failure. Methods: The SD rats were randomly divided into blank control group, heart failure model group, captopril group, and Low medium, and high doses of Jiawei Danshen Yin groups. The rat models of chronic heart failure were prepared by intraperitoneal injection of adriamycin solution for 4 weeks. The left ventricular ejection fraction (LVEF) of the rats was measured by echocardiography. The heart rate (HR), cardiac output (CO), systolic blood pressure (SBP), carotid artery diastolic pressure (DBF), left ventricular end diastolic pressure (LVEDP), left ventricular systolic pressure (LVSP) left ventricular pressure change maximum increase/decrease rate (±dp/dtmax) and other related hemodynamic parameters were measured by ventilator in each group to assess the rat heart function. The levels of inflammatory cytokines IL-6 and TNF-α were detected by enzyme-linked immunosorbent assay (ELISA). Results: Compared with blank control group, the LVEF of the rats in model group, captopril groups and low, medium, and high doses of Jiawei Danshen Yin groups were decreased (P<0. 05). Compared with model group, the LVEF of the rats in captopril group and low, medium, and high doses of Jiawei Danshen Yin groups were increased (P<0. 05). The HR, SBF, DBF, CO, LVSPt and ±dp/dtmax of the rats in model group were lower than those in blank control group (P<0. 05), while the LVEDP was increased ( Prats in captopril group and low, medium, and high doses of Jiawei Danshen Yin groups were increased ( P<0. 05), however the HR and LVEDP were decreased ( P<0. 05). Compared with low dose of Jiawei Danshen Yin group, the CO and ±dp/dtmax of the rats in captopril group and medium and high doses of Jiawei Danshen Yin groups were increased (P<0. 05) and the LVEDP were decreased (Pcontrol group, the levels of serum IL-6 and TNF-α of the rats in the other groups were increased ( P<0. 05). The levels of serum IL-6 and TNF-α of the rats in captopril group and low. medium, and high doses of Jiawei Danshen Yin groups were lower than those in model group (P<0. 05). Compared with low dose of Jiawei Danshen Yin group, the levels of serum IL-6 and TNF-α of the rats in captopril group and medium and high doses of Jiawei Danshen Yin groups were decreased (P<0. 05). Compared with blank control group, the indexes of heart and lung in the other groups were all increased ( P<0.05); compared with model group, the heart and lung indexes of the rats in captopril group and low, medium, and high doses of Jiawei Danshen Yin groups were decreased (P<0. 05).

Conclusion:

Jiawei Danshen Yin can improve the heart function of the rats with heart failure and inhibit the expressions of serum inflammatory cytokines IL-6 and TNF-α to some extent. In the common dose range, Jiawei Danshen Yin can maximize the benefit of cardiac function and the serum inflammatory factors in the rats with chronic heart failure.