Effect of siwutang on recovery of anemia induced by chemotherapy in mice and its mechanism / 吉林大学学报(医学版)

ABSTRACT

Objective: To study the recovery effect of Siwutang on the anemia induced by chemotherapy in the mice, and to clarify its mechanism. Methods: A total of 24 mice were randomly divided into control group (n=8, not given any drugs), model group (n= 8, given 5-fluorouracil), Siwutang group (n= 8, given 5-fluorouracil + Siwutang). After administration for 15 d, the peripheral blood of mice was taken, and the morphology and the number of red cells were observed under microscope. The number of peripheral blood red cells and the levels of hemoglobin of the mice in various groups were calculated by full-automatic hemocytometer; Double staining was used to mark the surface antigens of red cells, then antibody binding and staining were performed. Flow cytometry was used to detect the number of peripheral blood-labelled and spleen-labelled red cells. Results: Compared with control group, the number of red cells in peripheral blood of the mice in model group was significantly decreased (P<0. 05), and the cells shrinkaged. Compared with model group, the number of red cells in peripheral blood of the mice in Siwutang group was significantly increased (P<0. 05) and the cells showed rounding. Compared with control group, the level of hemoglobin in peripheral blood of the mice in model group was significantly decreased (P<0. 05); compared with model group, the level of hemoglobin in peripheral blood of the mice in Siwutang group was significantly increased (P<0. 01). The flow cytometry results showed that compared with control group, the expression level of CD71/Terll9 in peripheral blood of the mice in model group was significantly decreased (P<0. 01), and the number of red cells in various quadrants was decreased (P<0. 01); compared with model group, the expression level of CD71/Terll9 in peripheral blood of the mice in Siwutang group was significantly increased (P<0. 01), and the number of red cells in various quadrants was increased (P<0. 01); compared with control group, the expression level of CD71/Terll9 in spleen blood of the mice in model group was significantly decreased (P< 0. 01), and the number of cells in various quadrants was decreased (P<0. 01); compared with model group, the expression level of CD71/Terll9 in spleen blood of the mice in Siwutang group was significantly increased (P<0.01), the number of red cells in UL quadrant was decreased (P<0. 01), and the number of red cells in UR and LR quadrants was increased (P<0.01). Conclusion: Siwutang can promote the recovery of anemia induced by chemotherapy in the mice, increase the number of red cells, improve the morphology of red blood cells, increase the level of hemoglobin in peripheral blood, and increase the expression levels of marker antigens on the surface of red cells in the peripheral blood and spleen.