Enhancement of histone deacetylase inhibitors SAHA in chemotherapeutic sensitivity of ovarian cancer SKOV3 cells / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the biological effects of histone deacetylase inhibitors (HDACIs) suberoylanilide hydroxamic acid (SAHA) combined with vincristine (VCR) on the ovarian cancer SKOV3 cells, and to clarify their mechanisms. Methods: The human ovarian cancer SKOV3 cells were divided into control group, SAHA group, VCR group and SAHA+VCR group. CCK-8 method was used to detect the surival rates of SKOV3 cells in various groups. The apoptotic rates, the autophagy rates and the distribution of cell cycles of SKV03 cells in various groups were detected by flow cytometry (FCM). Results: The CCK-8 results showed that compared with control group, the survival rates of SKOV3 cells in SAHA group and VCR group were decreased significantly. Compared with VCR group or SAHA group, the survival rates of cells in SAHA + VCR groups were decreased significantly (Pcontrol group, the apoptotic rates, the autophagy rates and the percentages of the SKOV3 cells at G2/ M phase in SAHA group and VCR group were increased (P<0. 05); compared with SAHA or VCR groups, the apoptotic rate, the autophagy rate and the percentages of SKOV3 cells at G2/M phase in SAHA + VCR groups were increased (P < 0.05).

Conclusion:

SAHA can enhance the sensitivity of ovarian cancer SKOV3 cells to VCR and induce the apoptosis and autophagy of SKOV3 cells to promote the cell arrest at GI/ M phase. The combined application of SAHA and chemotherapeutic drugs may be a new strategy for the treatment of ovarian cancer patients.