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Effects of silencing expression of CRAF gene on invasion and metastasis of hepatocellular carcinoma cells / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition) ; (6): 521-525, 2018.
Artigo em Chinês | WPRIM | ID: wpr-841879
ABSTRACT

Objective:

To investigate the effects of high expression of CRAF on the invasion and metastasis of hepatocellular carcinoma cells (HCC), and to clarify their mechanisms.

Methods:

The SMMC7721 cells with high expression of CRAF were regarded as the object, and the expressions of CRAF in SMMC7721 cells were down-regulated by shRNA. The SMMC7721 cells were divided into control group and silencing groups (shCRAF# 1 transfection group and shCRAF# 2 transfection group). The wound closure index of SMMC7721 cells was detected by cell scratch test, the number of transmembrane cells of SMMC7721 cells was detected by Transwell assay, and the expression levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in SMMC7721 cells were detected by Western blotting method.

Results:

The scratch test results showed that compared with control group, the wound closure indexes of the SMMC7721 cells in silencing groups were decreased (P<0. 05). The Transwell assay results show that compared with control group, the number of transmembrane cells in silencing groups was decreased (P<0. 05). The Western blotting results showed that compared with control group, the expression levels of MMP-2 and MMP-9 in the SMMC7721 cells in silencing group were decreased (P<0. 05).

Conclusion:

Silencing CRAF can inhibit the invasion and migration of HCC SMMC7721 cells by inhibiting the expressions of MMP-2 and MMP-9.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Jilin University(Medicine Edition) Ano de publicação: 2018 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Jilin University(Medicine Edition) Ano de publicação: 2018 Tipo de documento: Artigo