Relationships between expressions of B7-H1 and B7-H4 and Foxp3+ regulated T-cell infiltration in colorectal cancer tissue / 吉林大学学报(医学版)

ABSTRACT

Objective: To explore the expressions of B7-H1 and B7-H4 in colorectal cancer (CRC) tissue and their relationships with the Foxp3 regulated T-cell (Treg) infiltration in tumor tissue, and to provide the basis for judging the biological behaviours and prognosis of CRC. Methods: Immunohistochemistry was used to detect the expressions of B7-H1 and B7-H4 and the infiltration of Treg in 56 cases of CRC tissue samples and adjacent normal tissue samples, and their relationships with the clinicopathological features of CRC patients were analyzed. Results: The positive expression rate of B7-H1 in CRC tissue of the CRC patients was higher than that in adjacent normal tissue (χ2= 72. 34, P<0. 01). The differences of expression frequencies of B7-H1 in CRC tissues with different infiltration depths were significant (P<0. 01). The high expression frequency of B7-H1 in CRC tissue of the patients with positive lymphnode metastasis was higher than that in the patients with negative lymphnode metastasis (P<0. 01). The high expression frequency of B7-H1 in CRC tissue of the patients at Duke' s stage (C+D) was higher than that of the patients at Duke' s stage (A+B) (P<0. 01). The positive expression rate of B7-H4 in CRC tissue was higher than that in adjacent normal tissue (χ2=78. 92, P<0. 01). The differences of expression frequencies of B7-H4 in CRC tissues with different infiltration depths were significant (P<0. 05). The high expression frequency of B7-H4 in CRC tissue of the patients with positive lymphnode metastasis was higher than that of the patients with negative lymphnode metastasis (P<0. 05). The number of Treg positive expression cells in CRC tissue was significantly higher than that in adjacent normal tissue (P<0. 01), the number of Treg postive expression cells in CRC tissues with different differentiation degrees were different (P<0. 01), and the number of Treg positive expression cells in CRC tissue of the patients with positive lymphnode metastasis was higher than that of the patients with negative lymph node metastasis (P<0. 01). The expression of B7-H1 was correlated with Treg infiltration (P<0. 01, Cramer ' s = 0. 463); the expression of B7-H4 was correlated with Treg infiltration (P<0. 05, Cramer' s = 0. 320). Conclusion: B7-H1 and B7-H4 are highly expressed in human CRC tissue, and they are associated with the increasing of Treg infiltration; they can be used as the indicators to judge the prognosis of CRC.