Comparsion of GV oocytes transcriptome between PCOS patients and normal ovulatory women / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition)
;
(6): 568-573, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-841888
ABSTRACT
Objective:
To investigate the effects of controlled ovarian stimulation (COS) on the differentially expressed genes in GV oocytes and main signal transduction pathways in the polycystic ovary syndrome (PCOS) patients and the normal ovulatory women, and to screen the key genes impacting the development of oocytes of the PCOS patients.Methods:
During controll ovarian hyperstimulation with GnRH-a long protocol, 3 patients with PCOS (PCOS group) and 3 normal ovulatory women due to male infertility factor (control group) were selected. Enzyme digestion was used to islolate the granule cells. The dumped immature oocytes were collected after intracytoplasmic sperm injection (ICSI). The cDNA library was constructed and sequencing was performed in Illumina MiSeq sequencing platform and RT-PCR was used to confirm the data obtained in vivo.Results:
A total of 510 024 82 sequence reads were obtained, and 8 G base sequence information were contained. A total of 63 differentially expressed genes were found by bio-informatics software, including 19 significant up-regulation genes and 44 significant down-regulation genes (Fold Change>4, FDR<0. 01). The expression levels of vascular endothelial growth (VEGF) and fatly acid dehydrogenase 1 (FADS1) mRNA in the patients in PCOS group were significantly higher than those in control groups (PConclusion:
The differentially expressed genes in GV oocytes of the PCOS patients may affect the development of follicle and maturation of oocytes and lead to poor quality of embryo.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Jilin University(Medicine Edition)
Ano de publicação:
2018
Tipo de documento:
Artigo
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