UGT88B2: A promiscuous O-glycosyltransferase from Carthamus tinctorius / 中草药·英文版
Chinese Herbal Medicines
;
(4): 440-445, 2020.
Artigo
em Chinês
| WPRIM
| ID: wpr-841996
ABSTRACT
Objective:
In order to obtain new glycosyltransferases with highly efficient catalysis, the glycosyltransferases from Carthamus tinctorius which contains diverse types of glycosides were mined.Methods:
A new glycosyltransferase gene (UGT88B2) with full length was obtained by PCR and further transformed into Escherichia coli for heterologous expression. The catalytic activity of recombinant UGT88B2 was determined by HPLC-MSn. The structures of representative catalytic products were elucidated by MS and NMR.Results:
UGT88B2 exhibited catalytic promiscuity and various patterns in glycosylation of flavonoids with high efficiency.Conclusion:
A new glycosyltransferase named UGT88B2 was successfully mined and can be employed as enzymatic tools in glycosylation of flavonoids.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Herbal Medicines
Ano de publicação:
2020
Tipo de documento:
Artigo
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