Effect of indoxyl sulfate on proliferation and expression of inflammatory cytokines and ROS in human periodontal ligament cells / 上海交通大学学报（医学版）

ABSTRACT

Objective: To explore the effects of Indoxyl sulfate (IS) on proliferation activity, expression of inflammatory factors and reactive oxygen species (ROS) in human periodontal ligament cells (hPDLCs). Methods ¡¤ The primary hPDLCs were cultured by using tissue explant method in vitro. MTT assay was employed to evaluate the effect of IS on proliferation activity of hPDLCs. The mRNA and protein expressions of IL-1β, IL-6, and IL-8 were detected by using real-time PCR and ELISA assay. DCFDA fluorescence probe was used to detect intracellular ROS expression and ROS in the cytoplasm under fluorescence microscope. Results ¡¤ The viability of hPDLCs was inhibited by IS at the concentra-tion of 125 μmol/L on 24, 48 and 72 hours. The inhibitory effect was presented in a dose- and time- dependent manner. IS could upregulate the mRNA and protein expression of inflammatory cytokines including IL-1β, IL-6 and IL-8 as well as promote ROS in hPDLCs. Conclusion ¡¤ IS may play an important role in the association between chronic kidney diseases and periodontitis through inhibiting the activity of hPDLCs, pro-moting the expression of inflammatory cytokines and increasing intracellular ROS level.