IL-17A inhibits degradation of ABCA1 protein through p38MAPK in macrophages / 西安交通大学学报(医学版)
Journal of Xi'an Jiaotong University(Medical Sciences)
;
(6): 853-856, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-843937
ABSTRACT
Objective:
To investigate the underlying mechanisms and intracellular signaling of up-regulating adenosine triphosphate binding cassette transporter A1 (ABCA1) by interleukin-17A (IL-17) in macrophages.Methods:
Mouse RAW264.7 cells were treated with 20ng/mL of IL-17A. The protein expressions of ABCA1 and p-p38MAPK were detected using Western blot. The ubiquitination level of ABCA1 was detected using Co-immunoprecipitation.Results:
IL-17A increased the expression of ABCA1 at the protein level, but not at the mRNA level. IL-17A reduced the degradation of ABCA1 protein. IL-17A attenuated binding of ABCA1 to ubiquitin. SB203580, p38MAPK inhibitor, reversed the decrease of ubiquitination and increased expression of ABCA1 protein induced by IL-17A in macrophages.Conclusion:
IL-17A increases the expression of ABCA1 protein by inhibiting its degradation through p38MAPK pathway in macrophages.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Xi'an Jiaotong University(Medical Sciences)
Ano de publicação:
2019
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS