Expression of HK2 in ovarian cancer tissue and its effects on proliferation of ovarian cancer cells / 西安交通大学学报(医学版)

ABSTRACT

Objective: To explore the expression of hexokinase 2 (HK2) in ovarian cancer tissues and its effect on the proliferation of ovarian cancer cells. Methods: The levels of HK2 in cancer tissues of 27 patients and normal tissues of 14 patients were detected with qRT-PCR and immunohistochemical analysis. Overexpression of HK2 by transfected PLHKII-pGFPN3 plasmid and knockdown of HK2 by transfected HK2 siRNA were used in our study. Proliferation of SKOV3 cells was detected by CCK8 method. The expression of HK2 was measured by Western blot. Results: The expression of HK2 in 27 patients with ovarian cancer (92.6%, 25/27) was up-regulated compared with normal tissues (42.9%, 6/14). The expression of HK2 in the cancer tissues of patients with clinical stage III-, pathological grade G3, and distant metastasis was up-regulated significantly (P<0.05). The growth of SKOV3 cells was significantly promoted by overexpression of HK2, and the growth of SKOV3 cells was inhibited by downexpression of HK2. Conclusion: The expression of HK2 is significantly higher in ovarian cancer tissues than in normal ovarian tissues. HK2 can promote the proliferation of ovarian cancer cells.