Effects of down-regulation of astrocyte elevated gene-1 expression by siRNA on glioma vasculogenic mimicy / 西安交通大学学报(医学版)

ABSTRACT

Objective: To investigate the effects of down-regulation of astrocyte elevated gene-1(AEG-1) expression by siRNA on glioma vasculogenic mimicy in vitro. Methods: The expression of AEG-1 in U87 glioma cells was down-regulated by siRNA. An in vitro model of glioma vasculogenic mimicy was established to evaluate the effect of down-regulation of AEG-1 expression on glioma vasculogenic mimicy. Real-time PCR and Western blot were used to detect the mRNA and protein expressions of VEGF and MMP2 in U87 cells after treated with AEG-1 siRNA, respectively. Immunofluorescence was used to detect the expressions of VEGF and MMP2 in glioma vasculogenic mimicy model in vitro. Results: Down-regulation of AEG-1 expression by siRNA could significantly inhibit the glioma vasculogenic mimicy in vitro(P<0.01). Meanwhile, It could also inhibit the expressions of VEGF and MMP2 in glioma cells and glioma vasculogenic mimicy in vitro (P<0.01). Conclusion: These results suggest that down-regulation of AEG-1 expression by siRNA can significantly inhibit the glioma vasculogenic mimicy in vitro, and these effects may be realized partly through regulation of VEGF and MMP2 expression.