Enhancing effect of Lupeol on migration and invasion abilities of ST3Gaim-silenced MDA-MB-231 cells / 解剖学报

ABSTRACT

Objective In this study, we expored the enhancing effect of Lupeol on migration and invasion abilities of ST3Gal III-silenced MDA-MB-231 cells. Methods Human breast cancer cell line ST3Gal III -silenced MDA-MB-231 was cultured in vitro. The cell adhesion, Transwell and woud healing test were utilized to test the effect of Lupeol on ST3GalM-silenced MDA-MB-231 cells. The expression of matrix metalloproteinase( MMP)-2, -9, phosphatidylinositol 3-kinase/protein kinase B/nuclear factor κB ( PI3K/Akt/NF-KB) signaling pathway were examined by Western blotting. Results No significant influence of the decreased expression of ST3Gal III and Lupeol ( 5μmol/L) on proliferation and apoptosis of MDA-MB-231 cells were found. Lupeol inhibited the migration and invasion of ST3Gal III -silenced MDA-MB-231 cells in vilro( P<0. 05). Furthermore, the expression of NF-κB p65, p-Akt, Akt, p85, MMP-2 and MMP-9 levels were significantly down-regulated. Conclusion These observations suggest that Lupeol may inhibit the abilities of migration and invasion of ST3Gal III-silenced MDA-MB-231 cells in vitro by inhibiting the protein expression of MMP-2, -9 and effect the signaling pathway of PI3K/Akt/NF-κB.