Genetic polymorphism of rsl051169 G/C and rs9984765 T/C in S100B gene in Chinese Guangxi population / 解剖学报

ABSTRACT

Objective: To explore the distribution characteristics of S100B gene rsl051169 G/C and rs9984765 T/C polymorphisms in Guangxi population, and to compare the distribution differences among different races and districts population. Methods: Polymerase chain reaction-single base extension (SBE-PCR) and DNA sequencing were used to detect the genotype of rslOSl 169 G/C and rs9984765 T/C among 398 individuals in Guangxi. The results were compared with the allele and genotype of other four populations (HapMap-CEU, HapMap-YRI, HapMap-JPT, HapMap-HCB) from Haplotype Map(Hap Map). Results: There were GG, CG and CC genotypes at the rs 1051169 G/C of S100B gene in Guangxi population, with frequencies of 41.2%, 44.7% and 14. 1%, respectively, and G and C allele frequencies were 63.6% and 36.4%, respectively. TT, CT and CC genotypes were found at rs9984765 T/C, with frequencies of 47. 7%, 44.5% and 7. 8%.respectively, and allele frequencies of T and C were 70.0% and 30. 0%, respectively. There were no significant differences in genotype and allele frequencies of rsl051169 G/C and rs9984765 T/C among male and female in Guangxi population (P>0. 05). The genotype and allele frequency of rs 1051169 G/C in Guangxi population showed significant difference as compared with HapMap-CEU, HapMap-YRI and HapMap-JPT (P0. 05). The genotype and allele frequency of rs9984765 T/C in Guangxi population showed significant difference as compared with HapMap-YRI, HapMap- JPT and HapMap-HBC (1 0. 05). Conclusion: The polymorphisms of rs 1051169 G/C and rs9984765 T/C in S100B gene in Guangxi populations, and their polymorphisms are different from different races and district populations.