Determination of enantiomeric impurity of etomidate by high performance liquid chromatography / 西安交通大学学报·英文版

ABSTRACT

Objective: To determine enantiomeric impurity of etomidate using high performance liquid chromatography. Methods: (R)-etomidate and (S)-etomidate were separated on a CHIRALPAK AD-H column. The mobile phase consisted of 20 : 80(v/v) isopropanol-n-hexane. The flow rate of the mobile phase was 0.5 mL/min. The detected wavelength was 242 nm. Results: (R)-etomidate and (S)-etomidate could be separated completely under these conditions. The precision of (R)-etomidate was 1.57% (n = 3). The limit of detection of (R)-etomidate was 4. 25 ng/mL. The average percentage content of (S)-etomidate was 0.09% in the samples. Conclusion: The method was repeatable and sufficiently sensitive to determine the enantiomeric impurity of etomidate. It allows the quantitation of the impurities at the 0.085% (w/w) level relative to etomidate at a concentration of the test solution of 5 mg/mL.