Effects of Panax notoginseng saponins on hydrogen peroxide-induced apoptosis in cultured rabbit bone marrow stromal cells / 西安交通大学学报·英文版

ABSTRACT

Objective: To investigate the effects of Panax notoginseng saponins (PNS) on hydrogen peroxide (H2O2)-induced apoptosis in cultured rabbit bone marrow stromal cells (BMSCs). Methods: BMSCs from 3-month-old New Zealand rabbits were isolated and cultured by the density gradient centrifugation combined with adherent method. The cultured BMSCs were divided into three groups normal control, H2O2 treatment (100 μmol/L), and PNS pretreatment (0.1 g/L). Intracellular reactive oxygen species (ROS) levels as the index of oxidative stress were measured by using 2′7′-dichlorodihydrofluorescein diacetate. Flow cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/PI. The protein expression of Bax in BMSCs was analyzed by Western blotting. Activity of caspase-3 enzyme was measured by spectrofluorometry. Results: Pretreatment with PNS significantly decreased intracellular ROS level induced by H 2O2 (P<0.01). PNS markedly attenuated H 2O2-induced apoptosis rate from 38.68% to 19.24% (P<0.01). PNS reversed H2O2-induced augmentation of Bax expression. Furthermore, PNS markedly reduced the altered in activity of caspase-3 enzyme induced by H2O2 (P<0.01). Conclusion: PNS has a protective effect on hydrogen peroxide-induced apoptosis in cultured rabbit BMSCs by scavenging ROS and decreasing Bax expression and caspase-3 activity.