Simultaneous determination of nine ingredients in Shenkangling granules by HPLC wavelength switching method / 国际药学研究杂志

ABSTRACT

Objective: To establish an HPLC wavelength switching method for the simultaneous content determi- nation of verbascoside,paederosidic acid methyl ester,asperulosidic acid,asperuloside,calycosin7-O-β-D-glucopyran- oside,ononin,calycosin,formononetin and batatasinin Shenkangling granules(Radix Rehmanniae,Herba Hedyotis Diffusae,Radix Astragali,Rhizoma Dioscoreae,etc.). Methods: The Shenkangling granules were extracted with 50% aqueous methanol to prepare the test solution. The HPLC analysis was performed on thermostatic Agilent Eclipse XDB-C18 (250 mm×4.6 mm,5 μm)a 30℃,with the mobile phase consisting of acetonitrile-0.2% phosphoric acid solution at 0.9 ml/min flow rate in a gradient elution manner,and the detection wavelength was set at 334,238,254 and 270 nm, respectively. Results The above mentioned nine compounds showed good linearity in turn within the range of 3.09- 61.80,3.67-73.40,1.98-39.60,2.51-50.20,1.39-27.80,1.06-21.20,0.87-17.40,2.79-55.80,and 2.16-43.20 μg/ml, respectively,and their average recovery was 99.34%,100.03%,97.67%,98.63%,97.96%,96.93%,97.48%,99.87%, and 98.95% with the RSD of 0.88%,1.15%,1.28%,1.49%,0.99%,1.41%,1.37%,0.72%,and 1.25%,respectively.Conclusion: The method is easy to operate,repeatable,and can be used for quality control of Shenkangling granules.