A transcriptomic study on cerebral ischemia rat model / 国际药学研究杂志

ABSTRACT

Objective: To study the differences in gene expression between cerebral ischemia rats and normal rats. Methods: Sixteen male rats were randomly divided into normal group and model group. Rat left cerebral ischemic model was prepared by thread embolism, and RNA-seq sequencing was performed. Results: There were 530 differential genes between the two groups, including 37 significant difference genes and 98 extremely significant difference genes. After gene ontology(GO)analysis, it was found that the biological processes and the molecular functions of some related genes showed an upward trend. In the genes associated with cellular components, there were both a significant up-regula- tion trend and a significant down-regulation trend;the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway anal- ysis revealed that the genes related to P53 signaling pathway were significantly down-regulated, while the genes related to steroid hormone biosynthetic pathways were significantly up-regulated. Conclusion: The formation of cerebral ischemia was likely to be closely related to the apoptosis and postsynaptic density, and the down-regulation of P53 signal pathway and up-regulation of steroid hormone biosynthesis pathway could affect the formation of cerebral ischemia.