Effects of Gualou Xiebai dropping pills against myocardial ischemia reperfusion injury / 国际药学研究杂志

ABSTRACT

Objective To verify the protective effects of Gualou Xiebai dropping pills(GX) on myocardial ischemia-reperfusion injury(MIR.I) in rats. Methods After screening the qualified SPF rats were randomly divided into four groups, with 12 in each group. Sham-operated group and model group rats were respectively treated with normal saline, and rats in GX group and compound Danshen dropping pills group were given the corresponding dropping pills equivalent to 22.5 g/kg and 85.05 mg/kg of the crude herb, respectively. All groups were administered once a day for 7 successive days. One hour after the last administration, the MIRI models were produced by occluding the left coronary artery for 30 min and releasing the occlusion for 120 min. The changes in ST segment were observed, and the contents of creatine kinase-MB (CK-MB), cardiac troponin-T (CTNT) and myoglobin (MYO) in blood plasma were measured. The pathological changes in myocardial tissues were observed by optics and electric-microscope and the percentage of myocardial infarction was measured by detecting the content of Evan blue in myocardium. Results Compared to normal saline in the model group, GX had antagonism to ECG S-T segments elevation in rats with myocardial ischemia, while the contents of CK-MB, MYO, and CTNT in blood plasma declined significantly (P<0.05 or P<0.01); the disorder condition of myocardial cell fiber arrangement was improved, and edema between myocardial tissue and cells was relieved; the inflammatory cell infiltration and myocardial necrosis in cardiac allograft were significantly alleviated, and the percentage of myocardial infarction was decreased significantly (P< 0.01). Conclusion GX may play an important protective role against the MIRI in rats.