Pharmacokinetic comparison of paeoniflorin in rats under normoxic and hypoxic conditions / 国际药学研究杂志

ABSTRACT

Objective To study and compare the pharmacokinetic characteristics of paeoniflorin in rats under hypoxic and normoxic conditions. Methods A highly effective and rapid ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method with multiple-reaction monitoring scanning via electrospray ionization source was successfully developed and validated for quantitation of paeoniflorin in rat plasma. A total of 12 SD rats were randomly divided into the hypoxic and normoxic groups. Each rat was received a single dose of paeoniflorin with 80 mg/kg through intragastric administration. The blood samples were drawn from the jugular veins to measure the plasma drug concentrations in rats at different time points. The pharmacokinetic parameters were processed by DAS software. The statistical analysis was carried out by using SPSS software according to independent sample t test. Results The main pharmacokinetic parameters of paeoniflorin between the hypoxia and the normoxic rats were AUC(0-t) 26 600.7 and 35 702.4 ng·min/ml, MRT(0-t) 127.2 and 109.7 min, T1/2 111.6 and 108.6 min, Tmax 50.8 and 35.0 min, and Cmax 176.9 and 332.7 ng/ml, respectively. The Cmax and AUC of paeoniflorin in hypoxia rats were significantly reduced, indicating that there was a statistical difference in in vivo drug level. Conclusion The pharmacokinetic behavior of paeoniflorin displayed some changes between the hypoxic and the normoxic rats. Current results provide some experiment basis to optimization and adjustment of dosage regimen for paeoniflorin preparation in clinical practice.