Effect of a novel sedative and hypnotic compound B2 on monoamine neurotransmitters levels in mouse brain / 国际药学研究杂志

ABSTRACT

Objective To study the effect of sedative and hypnotic compound B2 on levels of monoamine neurotransmitters, including norepinephrine (NE), dopamine (DA), and 5-hydroxytryptamine (5-HT) in mouse brain. Methods Mice were randomly divided into vehicle group and B2 group (5 mg/kg, ip), then the contents of NE, DA and 5-HT were detected by high performance liquid chromatography-electrochemical detection (HPLC-ECD). The effects of compound B2 on monoamine oxidase (MAO) activities in mouse hypothalamus, striatum and cortex were detected by colorimetric method. Results B2 (5 mg/kg, ip) could significantly reduce NE contoent in the hypothalamus from (1. 08 ± 0. 09) μgg to (0. 88 ± 0. 07) μgg (P < 0. 01) and reduce DA content in the striatum from (12. 85 ±2. 21) μgg to (8. 44 ± 2. 25) μgg (P < 0. 01). 5-HT content in hypothalamus were significantly decreased from (1. 12 ±0. 12) μgg to (0. 56 ±0. 18) μgg (P < 0. 01). There was no significant difference between two groups in MAO activity. Conclusion The sedative and hypnotic effects of B2 might be mediated by the decrease of monoamine levels in different mouse brain regions.