Change in expression of interleukin-17 in C57 mice’s spinal cord injury area / 国际药学研究杂志

ABSTRACT

Objective: To investigate the mechanism of expression of interlenkin (IL)-17 in C57 mice’s spinal cord clamp area, and to provide new targets for clinical treatment of spinal cord injury (SCI). Methods: Male C57BL/6 mice were randomly divided into three groups. In the spinal cord injury group, mice were made into spinal cord clamp model. In the sham surgery group, the dura was cut without injuring the spinal cord. The IL-17 neutralizing antibody group received IL-17 neutralizing antibody injection through the cadual vein at 1 hour after the spinal cord clamp . Mouse scale for locomotion(BMS) was applied to evaluate the mice’s behavior change of hindlimb in 1-7 days, the real time fluorescent quantitative PCR was used to detect the change in the expression of spinal cord injury district TNF-α mRNA each time, HE staining was conducted to detect the morphological changes of spinal cord injury of the sham surgery group, the spinal cord injury group and the IL-17 neutralizing antibody group at the 7th days. Results: After spinal cord injury, the mice’s BMS score were 9 in the sham surgery group; in the model of spinal cord injury group, the mice’s BMS score were 0 on the 1st day, and 2.9 on the 7th day. In the IL-17 neutralizing antibody group, the mice’s BMS score were 0 on the 1st day, and 3.5 on the 7th day. The expression of IL-17 mRNA in the injury area peaked at the 3rd hour, which showed statistical difference when compared with sham surgery group (P0.05), and the expression of IL-17 mRNA reduced the lowest levels on the 7th day. The 7th day following spinal cord injury, mice’s spinal cord tissue was complete normal in the sham surgery group. In the spinal cord injury group, a large number of mice’s nerve cells were necrotic, a lot of cells formed vacuolated. In the IL-17 neutralizing antibody group, part of mice’s nuclear neurons were shrinking, cells formed vacuolated, but part of cells remained morphologically complete. Conclusion: IL-17 is involved in secondary immune inflammatory process of spinal cord injury, it may be targets for intervention in the treatment of spinal cord injury.