Detoxicating mechanism of cortex magnoliae officinalis and fructus aurantii immaturus combination based on metabonomic studies / 国际药学研究杂志

ABSTRACT

Objective Magnolia officinalis Rehd. et Wils. (Hou Po) is one of the most popular traditional Chinese medicine. It has been used to treat a wide variety of clinical diseases such as wind-stroke, headache, cold and heat, fright qi, blood mpediment and dead muscle. In this study, H NMR-based metabonomics approach was used to nvestigate the toxicity of Houpo and Houpo combined with Fructus Aurantii Immaturus (Zhi Shi). Methods Male Wistar rats were divided nto three groups: control, group of Houpuo, group of Houpo combined with Zhishi. Based on H NMR spectra of urine and serum from rats, partial least-squares discriminant analysis (PLS-DA) was performed to dentify different metabolic profiles. Liver and kidney histopathology examinations were also performed. Results PLS-DA scores plots demonstrated that the metabolic profile treated with Houpo alone was different from that of the control rats. The metabolic profile of the group treated with Houpo combined with Zhishi was similar with that of the control. Statistics results were confirmed by the histopathological examination. Conclusion The combination of Houpo and Zhishi would relieve the damage n iver and kidney caused by Houpo.