Construction of aseptic cultural system of Salvia miltiorrhiza and determination of active components by HPLC analysis / 中草药

ABSTRACT

Objective: The regeneration system of Salvia miltiorrhiza was constructed and contents of active components of the sterile materials were determined, so as to provide the basis for the rapid propagation and secondary metabolism regulation of S. miltiorrhiza. Methods: The optimum hormone ratio for inducing callus was screened by orthogonal test. The buds and roots induction were conducted to estabilish the tissue culture system. The contents of effective components were evaluated by HPLC analysis. Results: The suitable medium for callus induction was MS+6-BA (2.0 mg/L)+NAA (1.0 mg/L)+2,4-D (0.5 mg/L). The preferred enrichment medium of adventitious bud induction was MS+6-BA (2.0 mg/L)+NAA (1.0 mg/L). And rooting medium was 1/2 MS+NAA (0.5 mg/L). Seven active components in aseptic seedlings, callus, and regenerated seedlings could be detected with significant differences in different aseptic materials (P < 0.05). The contents of rosmarinic acid and salvianolic acid B were higher than the others. Conclusion The culture system of S. miltiorrhiza was successfully established, and vigor regenerated seedlings were also obtained. The accumulation of active components in the three sterile materials showed difference, laying a foundation for further study in S. miltiorrhiza.