Diversity and biomarker exploration of endophytic fungi communities in different varieties of Paridis Rhizoma / 中草药

ABSTRACT

Objective: The analysis of endophytic fungi diversity and the biomarkers in different varieties of Paridis Rhizoma were used to lay a foundation for the development of quality equivalence and identification and traceability technology. Methods: The amplicons of endophytic fungal community genes from three varieties of Paridis Rhizomaa were sequenced by using Illmina Hiseq high-throughput sequencing. Bioinformatic analysis was performed to study the diversity of flora and the difference of fungal community. Results: The α diversity of endophytic fungi from different varieties was different, but there was no significant difference in β diversity. The OTU of Paris fargesii var. petiolata (Baker ex C. H. Wright) in endophytic fungi was only 0.27%. The results of fungal community annotation showed that the three varieties were annotated to six identical phyla, but the composition ratios were different. Moreover, we explored the biomarker differences in three variants, and the Rhizophagus irregularis of Paris polyphylla through LEfSe analysis. is a biomarker with significant difference. Conclusion: The diversity change of endophytic fungi community of the three varieties was not significant, but the composition ratio was different. The results of this study provided the research basis for the development of quality equivalence and identification and traceability technology.