Effect of sanggenon C on pulmonary fibrosis induced by bleomycin in mice and potential mechanisms implicated / 中草药

ABSTRACT

Objective: To observe the effect of sanggenon C on pulmonary fibrosis induced by bleomycin and explore its possible mechanism. Methods: C57BL/6 mice were randomly divided into four groups control group, model group, sanggenon C (100 and 50 mg/kg) group, 20 mice for each group. The control group and model group were induced by intratracheal injection of saline and bleomycin, respectively. The administration was started on day 4 after the operation, and the respiratory function of the mice was detected after 28 d of continuous administration. The content of hydroxyproline in lung was measured. Pulmonary inflammation and activity of collagen in lung were observed by HE staining and Masson staining. The expression of TGF-β1 protein in lung was detected by immunohistochemistry and detection of the expression of α-SMA, NF-κB p65, p-NF-κB p65, type I collagen and type III collagen in lung was detected by Western-blotting. Results: Compared with model group, sanggenon C improved the respiratory function in mice with pulmonary fibrosis induced by bleomycin. It significantly decreased the content of hydroxyproline, alleviated the degree of inflammation, and reduced the deposition of collagen in lung. The expression of TGF-β1, α-SMA, NF-κB p65, p-NF-κB p65, type I collagen and type III collagen in lung was significantly reduced. Conclusion: Sanggenon C can significantly improve the pulmonary fibrosis and respiratory function induced by bleomycin in mice. The mechanism may be related to the inhibition of TGF-β1 overexpression and the decrease of the expression of inflammatory transcription factor NF-κB and phosphorylation.