Study on multi-index components of Tibetan medicine Bangna of Aconitum genus according to fingerprints and chemometrics / 中草药

ABSTRACT

Objective: To establish the chemical fingerprint and multi-index components determination of Tibetan medicine Bangna of Aconitum genus, and provide references for the formulation of quality standards of multi-base original medicinal materials and clinically safe medication. Methods: HPLC fingerprint of Bangna was established and evaluated by the similarity evaluation system of TCM. In addition, the content of the seven components of Bangna from 30 batches and the difference of chemical information between the two species of Bangna was investigated by principal component analysis and orthogonal partial least squares discrimination analysis (OPLS-DA) respectively. Results: A total of 17 common peaks were identified in the fingerprint that was established by the determination of 30 batches of Bangna, and seven components of which were identified with 12-epi-napelline, songorine, benzoylmesaconitine, benzoylaconine, mesaconitine, aconitine, 3-acetylaconitine. Based on similarity results, the fingerprint had good consistency between the same origin and minor diversity between the different sources. The results of principal component analysis and OPLS-DA showed that there were some differences in the content of seven components between the two species. Based on the results of OPLS-DA and t test, it could be determined that the contents of 12-epi-napelline and aconitine of Aconitum flavum were significantly higher than those in Aconitum pendulum (P < 0.01). Conclusion: The fingerprint and multi-component quantitative analysis methods were used for the quality and clinically safe medication control of Bangna in this paper is simple, easy to operate, and informative. Moreover, it is necessary to establish and improve the limit determination of diester alkaloids.