UHPLC-Q-Exactive-Obitriap MS for quantification of ten components in Fuzi Lizhong Decoction / 中草药

ABSTRACT

Objective: To establish a method for quantifying ten components in Fuzi Lizhong Decoction (FLD). Methods: Ultra high performance liquid chromatography-Q-Exactive Obitriap mass spectrometry was used to detect components under optimized conditions as follows Hypersil Gold C18 column (50 mm × 2.1 mm, 1.9 μm), column temperature 30 ℃, mobile phase methanol (A)-water (B), gradient elution conditions 0-6 min, 25%-80% A, 6-7 min, 80%-95% A, 7-8 min, 95%-100% A, 8-9 min, 100% A, flow rate 0.3 mL/min; Ion source electrospray, scan mode full scan (positive, negative), scan range m/z 200-700 (positive), 200-500 (negative), resolution 17 500, capillary temperature 300 ℃, spray voltage +4.0 kV, -3.5 kV, sheath gas flow rate 35 L/h, S-lens voltage 50 V. Results: Satisfactory linearity with correlation coefficient (r2) higher than 0.995 was achieved for each compound. The content of benzoyl aconitine, benzoylmesaconine, atractylenolide I, atractylenolide II and lobetyolin scanned under positive mode in FLD was 2.52, 0.11, 0.46, 1.75 and 5.8 μg/mL respectively, and that of emodin, glycyrrhizin, liguiritigenin, 8-gingerol, 10-gingerol scanned under negative mode in FLD was 0.35, 2.52, 0.98, 6.65 and 2.71 μg/mL, respectively. Conclusion: The developed liquid chromatography-mass spectrometry method was fastwith low limit of detection, which could provide methodological basis for quality control of FLD as well as quantification of these compounds.