Neuroprotective effect of ginsenoside Rg1 in transgenic mice brain with Alzheimer's disease / 中草药

ABSTRACT

Objective: To investigate the effects of ginsenoside Rg1 on cognitive impairment, oxidative stress injury, inflammation, accumulation of A beta and neuronal apoptosis in APP/PS1 mice. Methods: Morris water maze test was used to evaluate the cognitive function of APP/PS1 mice. The oxidative damage and inflammation related indexes of APP/PS1 mice in each group were measured by kit and Elisa. The apoptotic and apoptotic proteins of hippocampal neurons in each group were quantified by HE staining and Western blot. Results: The results showed that Rg1 could significantly improve the cognitive function of mice, reduce the accumulation of Aβ and the content of malondialdehyde (MDA), and increase the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT). In addition, Rg1 could significantly reduce the serum levels of TNF-α, INF-γ, IL-1β and IL-6 in APP/PS1 mice, and increase the level of IL-2, thereby inhibiting the neuroinflammatory response. HE staining showed that Rg1 could reduce the apoptotic state of hippocampal cells. Rg1 also significantly increased the expression of Bcl-2/Bax protein ratio, and reduced the expression of Caspase-3, Caspase-9 and Cyt-c protein in hippocampus. Conclusion: Rg1 can significantly improve oxidative stress, reduce inflammatory response, inhibit neuronal apoptosis and improve cognitive function in APP/PS1 mice.