Antibacterial properties of jiao da bio-magnesium scaffolds in vitro / 中国组织工程研究

ABSTRACT

BACKGROUND: It has been confirmed that some biomedical magnesium alloy products have antibacterial properties, but the specific antibacterial mechanism is still unclear. OBJECTIVE: To investigate the antibacterial properties of biomedical Jiao Da Bio-Magnesium scaffold in vitro and explore possible mechanism. METHODS: Jiao Da Bio-Magnesium porous scaffold material extract was prepared. As the most common bacteria causing orthopedic implants infection, Escherichia coli and Staphylococcus aureus were selected for testing. The bacteriostasis rate was quantitatively evaluated by contact culture of the extraction solution. The bacteriostasis performance of the material was qualitatively evaluated by observing the bacterial morphology through scanning electron microscope. The alkaline phosphatase, conductivity, potassium ion, nucleic acid and protein content in bacterial extracellular liquid environment were detected. The possible antibacterial mechanism of Jiao Da Bio-Magnesium porous scaffold material extract was preliminarily explored. RESULTS AND CONCLUSION: (1) The bacteriostasis rate of Jiao Da Bio-Magnesium porous scaffold extract cultured with Escherichia coli for 12 hours ranged from 56.23% to 79.72%, while the Staphylococcus aureus group ranged from 62.34% to 76.07%. (2) Under scanning electron microscope, wizened form, smaller volume and scarcer distribution were observed. (3) The material extract had no effect on the content of alkaline phosphatase in the extracellular environment of the two bacteria, but increased the electrical conductivity and potassium ion content in the extracellular environment of the two bacteria. (4) The material extract had no effect on the content of nucleic acid and protein in the extracellular environment of Escherichia coli, and increased the content of nucleic acid and protein in the extracellular environment of Staphylococcus aureus. (5) The material extract could inhibit the nucleic acid content of the two bacteria, but had no effect on the soluble protein content of Escherichia coli cells, and inhibited the synthesis of soluble protein in Staphylococcus aureus cells. (6) Results suggested that Jiao Da Bio-Magnesium porous scaffold material has certain antibacterial properties in vitro, and the inhibitory effect on Staphylococcus aureus is stronger than that on Escherichia coli. The possible antibacterial mechanism is speculated that it can change the permeability of bacterial cell membrane and affect the synthesis of bacterial nucleic acids and proteins.