Basic fibroblast growth factor induces osteogenic and angiogenic differentiation of rat bone marrow mesenchymal stem cell patches / 中国组织工程研究

ABSTRACT

BACKGROUND: At present, cell patch technology has been widely used in the repair of various tissues and organs such as periodontal ligament, cornea, heart, cartilage, and esophagus. However, the effect and mechanism of basic fibroblast growth factor (bFGF) on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cell patches are still unknown. It is of great significance for understanding how to integrate growth factor with tissueengineered cell patch technology for the final use in tissue engineering repairing. OBJECTIVE: To investigate the expression of angiogenesis-related factors in rat bone marrow mesenchymal stem cell patch during bFGF-induced osteogenic differentiation. METHODS: After isolation, purification and identification, rat bone marrow mesenchymal stem cell patches were constructed by continuous induction of vitamin C and divided into two groups bFGF group (20 µg/L bFGF+osteoinductive medium) and control group (osteoinductive medium). The expression of angiogenesis-related genes was detected by alizarin red staining at 7 and 14 days of osteogenic induction and by fluorescence quantitative PCR method at 10 days of osteogenic induction. RESULTS AND CONCLUSION: The results of alizarin red staining showed that the number of calcified nodules in the bFGF group was significantly higher than that in the control group. The expression of transforming growth factor Β1 mRNA in the bFGF group was significantly higher than that in the control group, while the expression of vascular endothelial growth factor, angiopoietin 1 and platelet-derived factor BB was lower than that in the control group. Together, these results demonstrate that bFGF can induce the osteogenic differentiation of rat bone marrow mesenchymal stem cell patches, and increase the expression of transforming growth factor Β1 in the late osteogenic stage.