Silicate-Cu/Mg bioactive ceramics promote the osteogenesis of osteoblasts / 中国组织工程研究

ABSTRACT

BACKGROUND: The use of silicate bioceramics as a tissue-engineered bone scaffold has poor ability to promote osteogenesis. Studies have shown that copper, magnesium, and other essential trace elements have obvious effects on the induction and stimulation of osteoblasts and hemangioblasts. OBJECTIVE: To investigate the effects of silicate bioactive ceramics with Cu and Mg on osteoblast proliferation and osteogenesis. METHODS: Cu-silicate bioceramics, Mg-silicate bioceramics, and Cu-Mg-silicate bioactive ceramics were prepared by the sol-gel method (molar ratio of both Cu and Mg in ceramics was 5%). Three experimental groups were CS-5Cu, CS-5Mg, CS-5Cu/5Mg groups. The silicate bioactive ceramics served as the control group (denoted as CS). X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy were used to characterize the samples. The surface crystallization of bioceramics was detected. Osteoblasts were co-cultured with four groups of ceramics for 24 hours. Osteoblast proliferation index, alkaline phosphatase secretion, osteopontin and osteocalcin gene expression, vinculin and actin protein expression were determined. RESULTS AND CONCLUSION: (1) The crystallization ability of different silicate bioceramic samples followed the order of CS-5Cu>CS>CS-5Cu/5Mg>CS-5Mg. (2) Osteoblast proliferation index followed the rule of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS. (3) Alkaline phosphatase secretion was in the order of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS. (4) Osteopontin and osteocalcin gene expression followed the rule of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS. (5) Vinculin and actin protein expression was in the order of CS-5Cu/5Mg>CS-5Cu≈CS-5Mg>CS. (6) These results suggest that Cu- or Mg-silicate, in particular Cu-Mg-silicate bioactive ceramics can promote the proliferation of osteoblasts and the expression of osteogenesis-related genes as well as cell adhesion and spreading.