Mechanism of achyranthes bidentata bl.-taxillus Chinensis danser in the treatment of osteoarthritis based on network pharmacology / 中国组织工程研究

ABSTRACT

BACKGROUND: Achyranthes bidentata Bl.-Taxillus chinensis Danser is the most commonly used drug combination for clinical treatment of osteoarthritis, but its pharmacological mechanism is not clear. OBJECTIVE: To screen the main active chemical constituents of Achyranthes bidentata Bl.-Taxillus chinensis Danser based on the method of network pharmacology, collect the targets corresponding to the compounds, and build a drug-active ingredient-potential target network for treating osteoarthritis to treat bones and joints, and to systematically elucidate the pharmacological mechanism of inflammation. METHODS: The TCMSP database was used to obtain the active ingredients and targets of Achyranthes bidentata Bl.-Taxillus chinensis Danser, and the targets of osteoarthritis diseases obtained from the DisGeNET and GENE databases were intersected to screen out potential targets for the treatment of osteoarthritis by Achyranthes bidentata Bl.-Taxillus chinensis Danser. Using the String database and Cytoscape 3.6.1 software, we built a drug-active ingredient-therapeutic osteoarthritis potential target network and a protein-protein interaction network between potential targets. At the same time, the gene ontology (GO) biological function and Kyoto Encyclopedia of Genes and Gnomes (KEGG) pathway enrichment of potential targets were analyzed through the Omicshare cloud platform and DAVID database. RESULTS AND CONCLUSION: A total of 21 active ingredients and 48 potential targets for the prevention and treatment of osteoarthritis were screened from Achyranthes bidentata Bl.-Taxillus chinensis Danser. A total of 1 970 GO entries were obtained by GO enrichment analysis, of which 1 862 were biological processes, 29 were cellular components, and 79 were molecular functions (P < 0.01). KEGG pathway enrichment analysis screened out 76 potential signaling pathways for Achyranthes bidentata Bl.-Taxillus chinensis Danser to prevent osteoarthritis (P < 0.01). These findings indicate that Achyranthes bidentata Bl.-Taxillus chinensis Danser affects multiple metabolic pathways, affects different metabolic pathways that interacts and coordinates with each other, thereby preventing and curing osteoarthritis. This study tentatively explains the mechanism of the prevention and treatment of osteoarthritis by multi-component-multi-target-multi-pathway in Achyranthes bidentata Bl.-Taxillus chinensis Danser, which provides new ideas and clues for the prevention and treatment of osteoarthritis.