CIRBP gene up-regulated expression inhibits on the proliferation and migration of renal cancer and its possible mechanis / 肿瘤

ABSTRACT

Objective: To investigate the expression and biological functions of cold inducible RNA binding protein (CIRBP) in renal cancer. Methods: Bioinformatics analysis of microarray in Gene Expression Omnibus (GEO) and gene sequencing data in The Cancer Genome Atlas (TCGA) was used to analyze the expression of CIRBP mRNA in renal cancer, further the expression level of CIRBP gene in 20 cases of renal cancer tissues and 3 kinds of renal cancer cell lines was identified by real-time fluorescent quantitative PCR. The renal cancer 786-O and CAKI-1 cells were transfected with the CIRBP overexpression plasmids, then the cell proliferation viability was detected by MTT assay and cell clone formation assay. The migration ability of renal cancer cells was detected by Transwell chamer, and the expressions of cell migration-related protein N-cadherin, E-cadherin and protein kinase B (PKB, also known as AKT) pathway-related proteins were detected by Western blotting. Results: The expression level of CIRBP in renal cancer tissues was significantly lower than that in the adjacent normal tissues. The prognosis of patients with high expression of CIRBP mRNA was significantly better than that of the patients with low expression of CIRBP. The proliferation and clone formation of renal cancer 786-O and CAKI-1 cells transfected with CIRBP overexpresion plasmids were significantly inhibited (all P 0.01). The number of renal cancer 786-O and CAKI-1 cells migrated through the membrane in CIRBP overexpression group was less than that in the control group (all P 0.01). In the 786-O and CAKI-1 cells with CIRBP overexpression, the expression level of migration-related protein N-cadherin was significantly decreased, the expession level of E-cadherin was significantly increased, while the expressions of AKT pathwayrelated phospho-AKT (p-AKT) and phospho-glycogen synthasc kinase 3β (p-GSK3β) proteins were decreased significantly (all P 0.05). Conclusion: CIRBP is down-regulated in renal cancer, and inhibits the proliferation and migration of renal cancer cells. CIRBP can be used as a potential clinical diagnosis target and prognostic marker for renal cancer.