Expression of protocadherin 10 in non-small cell lung cancer and effect of protocadherin 10 over-expression on function of A549 cells / 肿瘤

ABSTRACT

Objective: To investigate the expression of protocadherin 10 (PCDH10) in non-small cell lung cancer (NSCLC), and to explore the function and molecular mechanism of PCDH10 in NSCLC cells. Methods: The expression levels of PCDH10 and Ki67 mRNAs in NSCLC tissues and paracancerous normal tissues were detected by real-time fluorescent quantitative PCR. The correlations of PCDH10 and Ki67 mRNA expressions with the clinicopathological features and prognosis of NSCLC patients were analyzed. A549 cells were transfected with recombinant plasmid pcDNA3.1(+)-PCDH10 or empty vector pcDNA3.1(+) (as the control) by LipofectAMINE 2000. The proliferation, apoptosis and cell cycle were detected by IncuCyte S3 live-cell analysis system and FCM method, respectively. The migration and invasion abilities were detected by Transwell method. The expression levels of proliferating cell nuclear antigen (PCNA), Bcl-2, cyclin D1, cyclin E, cyclin-dependent kinase 4 (CDK4), E-cadherin and Slug mRNAs and proteins were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. Results: The expression level of PCDH10 mRNA in NSCLC tissues was lower than that of para-cancerous normal tissues (P < 0.001). The expression level of PCDH10 mRNA in NSCLC tissues was negatively correlated with T stage, lymph node metastasis, clinical stage and Ki67 mRNA expression (all P < 0.001), and positively correlated with prognosis of NSCLC patients (P < 0.001). PCDH10 over-expression inhibited the proliferation of A549 cells by blocking cell cycle at G1 phase and promoting apoptosis (all P < 0.05). PCDH10 significantly inhibited the invasion and migration of A549 cells (both P < 0.05). The expression levels of PCNA, cyclin D1, cyclin E, CDK4, Bcl-2 and Slug mRNAs and proteins were downregulated in A549 cells with PCDH10 over-expression (all P < 0.05), but the expression levels of E-cadherin mRNA and protein were up-regulated (both P < 0.05). Conclusion: PCDH10 is lowly expressed NSCLC tissues. PCDH10 over-expression can inhibit the proliferation and invasion of NSCLC cells.