The expression of miR-98 in glioma and the promotion effect on invasion of glioma cells by miR-98 down-regulation / 肿瘤

ABSTRACT

Objective: To detect the expression of miR-98 in glioma tissues and investigate the effect of miR-98 on the invasion and migration of human glioma cell line U251. Methods: The expressions of miR-98 in glioma tissues and normal brain tissues were detected by real-time fluorescent quantitative PCR. After co-transfection with miR-98 inhibitor and wide type inhibitor of nuclear factor kappa B kinase epsilon (IKBKE) 3'-untranslated region (UTR) or mutation type IKBKE 3'-UTR recombination vector, the specific binding ability of miR-98 to 3'-UTR in IKBKE gene was examined by luciferase gene reporter system. The expression levels of miR-98, IKBKE mRNA and IKBKE protein in glioma cell line U251 after transfection with miR-98 inhibitor were measured by real-time fluorescent quantitative PCR and Western blotting, respectively.The abilities of migration and invasion of U251 cells after transfected with miR-98 inhibitor were detected by Transwell assay. The expression of miR-98 in U251 cells after transfection with IKBKE siRNA was detected by real-time fluorescent quantitative PCR. Results: The expression of miR-98 in glioma tissues was lower than that in normal brain tissues (P 0.05). Conclusion: The expression of miR-98 is low in glioma tissues. miR-98 inhibitor may promote the invasion and migration of glioma U251 cells by regulation of IKBKE expression.